PRS-01979hu Human Kynurenine (KYN) ELISA Kit

 Human Kynurenine (KYN) ELISA Kit

Purpose

This kit allows for the determination of KYN concentrations in Human serum, blood plasma, and other biological fluids.

Principle of the assay

The kit assay Human KYN level in the sample，use Purified Human KYN  antibody to coat microtiter plate wells, make solid-phase antibody, then add KYN  to wells, Combined KYN  antibody which With HRP labeled，become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of KYN  in the samples is then determined by comparing the O.D. of the samples to the standard curve.