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PRS-02719hu Human hydroxysteroid 11 beta dehydrogenase 1,11β-HSD1 Elisa kit

PRS-02719hu Catalogue No.
Reactivity
Human
Range
20pg/mL -600pg/mL
Sensitivity
3pg/mL
Catalogue NoSpecificationExw Reference Price( USD)Qty Avail
PRS-02719hu48T$268In store
PRS-02719hu96T$368In store


Human hydroxysteroid 11 beta dehydrogenase 111β-HSD1 Elisa kit


Purpose

This kit allows for the determination of 11β-HSD1 concentrations in Human serum, blood plasma, and other biological fluids.

Principle of the assay

The kit assay Human 11β-HSD1 level in the sampleuse Purified Human 11β-HSD1 antibody to coat microtiter plate wells, make solid-phase antibody, then add 11β-HSD1 to wells, Combined 11β-HSD1 antibody which With HRP labeledbecome antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 11β-HSD1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Materials provided with the kit

Materials provided with the kit

48determinations

96 determinations

Storage

User manual

1

1


Closure plate membrane

2

2


Sealed bags

1

1


Microelisa stripplate

1

1

2-8℃

Standard720pg/mL

0.5ml×1 bottle

0.5ml×1 bottle

2-8℃

Standard diluent

1.5ml×1 bottle

1.5ml×1 bottle

2-8℃

HRP-Conjugate reagent

3ml×1 bottle

6ml×1 bottle

2-8℃

Sample diluent

3ml×1 bottle

6ml×1 bottle

2-8℃

Chromogen Solution A

3ml×1 bottle

6ml×1 bottle

2-8℃

Chromogen Solution B

3ml×1 bottle

6ml×1 bottle

2-8℃

Stop Solution

3ml×1 bottle

6ml×1 bottle

2-8℃

wash solution

20ml×20 fold

×1bottle

20ml×30 fold

×1bottle

2-8℃