PRS-02719hu Human hydroxysteroid 11 beta dehydrogenase 1，11β-HSD1 Elisa kit

Human hydroxysteroid 11 beta dehydrogenase 1，11β-HSD1 Elisa kit

Purpose

This kit allows for the determination of 11β-HSD1 concentrations in Human serum, blood plasma, and other biological fluids.

Principle of the assay

The kit assay Human 11β-HSD1 level in the sample，use Purified Human 11β-HSD1 antibody to coat microtiter plate wells, make solid-phase antibody, then add 11β-HSD1 to wells, Combined 11β-HSD1 antibody which With HRP labeled，become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 11β-HSD1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Materials provided with the kit