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PRS-30205Ra Rat cyclooxygenase-2,COX-2 ELISA kit

PRS-30205Ra Catalogue No.
Reactivity
Rat
Range
0.5ng/ml - 150ng/ml
Sensitivity
0.5ng/ml
Catalogue NoSpecificationExw Reference Price( USD)Qty Avail
PRS-30205Ra48T$268In store
PRS-30205Ra96T$368In store



Rat cyclooxygenase-2,COX-2 ELISA kit


Purpose

This kit allows for the determination of COX-2 concentrations in Rat serum, blood plasma, and other biological fluids.

Principle of the assay

The kit assay Rat COX-2 level in the sampleuse Purified Rat COX-2 antibody to coat microtiter plate wells, make solid-phase antibody, then add COX-2 to wells, Combined COX-2 antibody which With HRP labeledbecome antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of COX-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Materials provided with the kit

Materials provided with the kit

48determinations

96 determinations

Storage

User manual

1

1


Closure plate membrane

2

2


Sealed bags

1

1


Microelisa stripplate

1

1

2-8℃

Standard180ng/ml

0.5ml×1 bottle

0.5ml×1 bottle

2-8℃

Standard diluent

1.5ml×1 bottle

1.5ml×1 bottle

2-8℃

HRP-Conjugate reagent

3ml×1 bottle

6ml×1 bottle

2-8℃

Sample diluent

3ml×1 bottle

6ml×1 bottle

2-8℃

Chromogen Solution A

3ml×1 bottle

6ml×1 bottle

2-8℃

Chromogen Solution B

3ml×1 bottle

6ml×1 bottle

2-8℃

Stop Solution

3ml×1 bottle

6ml×1 bottle

2-8℃

wash solution

20ml×20 fold

×1bottle

20ml×30 fold

×1bottle

2-8℃