PRS-30205Ra Rat cyclooxygenase-2,COX-2 ELISA kit

Rat cyclooxygenase-2,COX-2 ELISA kit

Purpose

This kit allows for the determination of COX-2 concentrations in Rat serum, blood plasma, and other biological fluids.

Principle of the assay

The kit assay Rat COX-2 level in the sample，use Purified Rat COX-2 antibody to coat microtiter plate wells, make solid-phase antibody, then add COX-2 to wells, Combined COX-2 antibody which With HRP labeled，become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of COX-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Materials provided with the kit