PRS-30015Ra Rat 6-keto-prostaglandin F1a,6-K-PGF1a ELISA Kit

Rat 6-keto-prostaglandin F1a,6-K-PGF1a ELISA Kit

Purpose

This kit allows for the determination of 6-K-PGF1a concentrations in Rat serum, blood plasma, and other biological fluids.

Principle of the assay

The kit assay Rat 6-K-PGF1alevel in the sample，use Purified Rat 6-K-PGF1a antibody to coat microtiter plate wells, make solid-phase antibody, then add 6-K-PGF1a to wells, Combined 6-K-PGF1a antibody which With HRP labeled，become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 6-K-PGF1a in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Materials provided with the kit