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PRS-01696hu Human fibroblast growth factor-21(FGF-21) ELISA Kit

PRS-01696hu Catalogue No.
Reactivity
Human
Range
4 ng/L-100 ng/L
Sensitivity
0.3 ng/L
Catalogue NoSpecificationExw Reference Price( USD)Qty Avail
PRS-01696hu48T$268In store
PRS-01696hu96T$368In store


Human fibroblast growth factor-21(FGF-21) ELISA Kit


Purpose

This kit allows for the determination of FGF-21 concentrations in Human serum, blood plasma, and other biological fluids.

Principle of the assay

The kit assay Human FGF-21 level in the sampleuse Purified Human FGF-21 antibody to coat microtiter plate wells, make solid-phase antibody, then add FGF-21 to wells, Combined FGF-21 antibody which With HRP labeledbecome antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of FGF-21 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Materials provided with the kit

Materials provided with the kit

48determinations

96 determinations

Storage

User manual

1

1


Closure plate membrane

2

2


Sealed bags

1

1


Microelisa stripplate

1

1

2-8℃

Standard135 ng/L

0.5ml×1 bottle

0.5ml×1 bottle

2-8℃

Standard diluent

1.5ml×1 bottle

1.5ml×1 bottle

2-8℃

HRP-Conjugate reagent

3ml×1 bottle

6ml×1 bottle

2-8℃

Sample diluent

3ml×1 bottle

6ml×1 bottle

2-8℃

Chromogen Solution A

3ml×1 bottle

6ml×1 bottle

2-8℃

Chromogen Solution B

3ml×1 bottle

6ml×1 bottle

2-8℃

Stop Solution

3ml×1 bottle

6ml×1 bottle

2-8℃

wash solution

20ml×20 fold

×1bottle

20ml×30 fold

×1bottle

2-8℃