PRS-01696hu Human fibroblast growth factor-21(FGF-21) ELISA Kit

Human fibroblast growth factor-21(FGF-21) ELISA Kit

Purpose

This kit allows for the determination of FGF-21 concentrations in Human serum, blood plasma, and other biological fluids.

Principle of the assay

The kit assay Human FGF-21 level in the sample，use Purified Human FGF-21 antibody to coat microtiter plate wells, make solid-phase antibody, then add FGF-21 to wells, Combined FGF-21 antibody which With HRP labeled，become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of FGF-21 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Materials provided with the kit