Purpose
This kit allows for the determination of ADP concentrations in Human serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Human ADP level in the sample，use Purified Human ADP antibody to coat microtiter plate wells, make solid-phase antibody, then add ADP to wells, Combined ADP antibody which With HRP labeled，become antibody-antigen- enzymeantibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ADP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
