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PRS-00820HU Human Insulin ELISA Kit

PRS-00820HU Catalogue No.
Reactivity
Human
Range
150ng/L -3500ng/L
Sensitivity
9ng/L
Catalogue NoSpecificationExw Reference Price( USD)Qty Avail
PRS-00820HU48T$268In store
PRS-00820HU96T$368In store



Human Insulin ELISA Kit


Purpose

This kit allows for the determination of Insulin concentrations in Human serum, blood plasma, and other biological fluids.

Principle of the assay

The kit assay Human Insulin level in the sampleuse Purified Human Insulin antibody to coat microtiter plate wells, make solid-phase antibody, then add Insulin to wells, Combined Insulin antibody which With HRP labeledbecome antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Insulin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Materials provided with the kit

Materials provided with the kit

48determinations

96 determinations

Storage

User manual

1

1


Closure plate membrane

2

2


Sealed bags

1

1


Microelisa stripplate

1

1

2-8℃

Standard4500ng/L

0.5ml×1 bottle

0.5ml×1 bottle

2-8℃

Standard diluent

1.5ml×1 bottle

1.5ml×1 bottle

2-8℃

HRP-Conjugate reagent

3ml×1 bottle

6ml×1 bottle

2-8℃

Sample diluent

3ml×1 bottle

6ml×1 bottle

2-8℃

Chromogen Solution A

3ml×1 bottle

6ml×1 bottle

2-8℃

Chromogen Solution B

3ml×1 bottle

6ml×1 bottle

2-8℃

Stop Solution

3ml×1 bottle

6ml×1 bottle

2-8℃

wash solution

20ml×20 fold

×1bottle

20ml×30 fold

×1bottle

2-8℃