PRS-00900hu Human Lactate Dehydrogenase,LDH ELISA Kit

Human Lactate Dehydrogenase,LDH ELISA Kit

Purpose

This kit allows for the determination of LDH concentrations in Human serum, blood plasma, and other biological fluids.

Principle of the assay

The kit assay Human LDH level in the sample，use Purified Human LDH antibody to coat microtiter plate wells, make solid-phase antibody, then add LDH to wells, Combined LDH antibody which With HRP labeled，become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of LDH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Materials provided with the kit