Rat hydroxyproline,Hyp ELISA Kit
Purpose
This kit allows for the determination of Hyp concentrations in Rat serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Rat Hyp level in the sample，use Purified Rat Hyp antibody to coat microtiter plate wells, make solid-phase antibody, then add Hyp to wells, Combined Hyp antibody which With HRP labeled，become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Hyp in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Materials provided with the kit
Materials provided with the kit | 48determinations | 96 determinations | Storage |
User manual | 1 | 1 |
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Closure plate membrane | 2 | 2 |
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Sealed bags | 1 | 1 |
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Microelisa stripplate | 1 | 1 | 2-8℃ |
Standard：27 μg/L | 0.5ml×1 bottle | 0.5ml×1 bottle | 2-8℃ |
Standard diluent | 1.5ml×1 bottle | 1.5ml×1 bottle | 2-8℃ |
HRP-Conjugate reagent | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
Sample diluent | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
Chromogen Solution A | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
Chromogen Solution B | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
Stop Solution | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
wash solution | （20ml×20 fold） ×1bottle | （20ml×30 fold） ×1bottle | 2-8℃ |
PRECISION
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/mean X 100
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Storage and validity
1．Storage： 2-8℃.
2．validity： 12 months.
