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PRS-30329Ra Rat hydroxyproline,Hyp ELISA Kit

PRS-30329Ra Catalogue No.
Reactivity
Rat
Range
1 μg/L-20 μg/L
Sensitivity
0.1 μg/L
Catalogue NoSpecificationExw Reference Price( USD)Qty Avail
PRS-30329Ra48T$268In store
PRS-30329Ra96T$368In store


Rat hydroxyproline,Hyp ELISA Kit



Purpose

This kit allows for the determination of Hyp concentrations in Rat serum, blood plasma, and other biological fluids.

Principle of the assay

The kit assay Rat Hyp level in the sampleuse Purified Rat Hyp antibody to coat microtiter plate wells, make solid-phase antibody, then add Hyp to wells, Combined Hyp antibody which With HRP labeledbecome antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Hyp in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Materials provided with the kit

Materials provided with the kit

48determinations

96 determinations

Storage

User manual

1

1


Closure plate membrane

2

2


Sealed bags

1

1


Microelisa stripplate

1

1

2-8℃

Standard27 μg/L

0.5ml×1 bottle

0.5ml×1 bottle

2-8℃

Standard diluent

1.5ml×1 bottle

1.5ml×1 bottle

2-8℃

HRP-Conjugate reagent

3ml×1 bottle

6ml×1 bottle

2-8℃

Sample diluent

3ml×1 bottle

6ml×1 bottle

2-8℃

Chromogen Solution A

3ml×1 bottle

6ml×1 bottle

2-8℃

Chromogen Solution B

3ml×1 bottle

6ml×1 bottle

2-8℃

Stop Solution

3ml×1 bottle

6ml×1 bottle

2-8℃

wash solution

20ml×20 fold

×1bottle

20ml×30 fold

×1bottle

2-8℃


PRECISION

 

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.

 

CV(%) = SD/mean X 100

Intra-Assay: CV<8%

Inter-Assay: CV<10%

 

 

Storage and validity

1Storage:  2-8℃.

2validity: 12 months.