PRS-00868hu Human Interleukin 33,IL-33 ELISA Kit

Product Name:Human Interleukin 33,IL-33 ELISA Kit  

Purpose

This kit allows for the determination of IL-33 concentrations in Human serum, blood plasma, and other biological fluids..

Principle of the assay

The kit assay Human IL-33 level in the sample，use Purified Human IL-33 antibody to coat microtiter plate wells, make solid-phase antibody, then add IL-33 to wells, Combined IL-33 antibody which With HRP labeled, become antibody -antigen-enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL-33 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Materials provided with the kit