PRS-00445hu Human Complement fragment 5b,C5b ELISA Kit

Product Name: Human Complement fragment 5b,C5b ELISA Kit 

Purpose

This kit allows for the determination of C5b concentrations in Human serum, blood plasma, and other biological fluids.

Principle of the assay

The kit assay Human C5b level in the sample，use Purified Human C5b antibody to coat microtiter plate wells, make solid-phase antibody, then add C5b to wells, Combined C5b antibody which With HRP labeled，become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of C5b in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Materials provided with the kit