PRS-00875hu Human Interleukin35,IL-35 ELISA Kit

Product Name: Human Interleukin35,IL-35 ELISA Kit  

Purpose

This kit allows for the determination of IL-35 concentrations in Human serum, blood plasma, and other biological fluids.

Principle of the assay

The kit assay Human IL-35 level in the sample，use Purified Human IL-35 antibody to coat microtiter plate wells, make solid-phase antibody, then add IL-35 to wells, Combined IL-35 antibody which With HRP labeled，become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL-35 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Materials provided with the kit