PRS-01719hu Human epididymis protein 4,HE-4 ELISA kit

Product Name:Human epididymis protein 4,HE-4 ELISA kit 

Purpose

This kit allows for the determination of HE-4 concentrations in Human serum, blood plasma, and other biological fluids.

Principle of the assay

The kit assay Human HE-4 level in the sample，use Purified Human HE-4antibody to coat microtiter plate wells, make solid-phase antibody, then add HE-4 to wells, Combined HE-4 antibody which With HRP labeled，become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of HE-4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Materials provided with the kit