PRS-01768hu Human reactive oxygen species(ROS) ELISA Kit

Product Name : Human reactive oxygen species(ROS) ELISA Kit 

Purpose

This kit allows for the determination of ROS concentrations in Human serum, blood plasma, and other biological fluids.

Principle of the assay

The kit assay Human ROS level in the sample，use Purified Human ROS antibody to coat microtiter plate wells, make solid-phase antibody, then add ROS to wells, Combined ROS antibody which With HRP labeled，become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ROS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Materials provided with the kit