PRS-01889hu Human Coenzyme Q10 (CoQ10) ELISA Kit

Product Name:Human Coenzyme Q10 (CoQ10) ELISA Kit  

Purpose

This kit allows for the determination of CoQ10 concentrations in Human serum, blood plasma, and other biological fluids.

Principle of the assay

The kit assay Human CoQ10 level in the sample，use Purified Human CoQ10 antibody to coat microtiter plate wells, make solid-phase antibody, then add CoQ10 to wells, Combined CoQ10 antibody which With HRP labeled，become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CoQ10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Materials provided with the kit