PRS-02674hu Human Glutathione peroxidase(GPX) ELISA Kit

Product Name:Human Glutathione peroxidase(GPX) ELISA Kit 

Purpose

This kit allows for the determination of GPX concentrations in human serum, blood plasma, and other biological fluids.

Principle of the assay

The kit assay human GPX level in the sample，use Purified human GPX antibody to coat microtiter plate wells, make solid-phase antibody, then add GPX to wells, Combined GPX antibody which With HRP labeled，become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GPX in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Materials provided with the kit