PRS-00871hu Human Interleukin 6 (IL-6) ELISA Kit

Product Name: Human Interleukin 6 (IL-6) ELISA Kit 

Purpose

This kit allows for the determination of IL-6 concentrations in Human serum, blood plasma, and other biological fluids..

Principle of the assay

The kit assay Human IL-6 level in the sample，use Purified Human IL-6 antibody to coat microtiter plate wells, make solid-phase antibody, then add IL-6 to wells, Combined IL-6 antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL-6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Materials provided with the kit