PRS-01541hu Human transforming growth factor-β1,TGF-β1 ELISA Kit

Purpose

This kit allows for the determination of TGF-β1 concentrations in Human serum, blood plasma, and other biological fluids..

Principle of the assay

The kit assay Human TGF-β1 level in the sample，use Purified Human TGF-β1 antibody to coat microtiter plate wells, make solid-phase antibody, then add TGF-β1 to wells, Combined TGF-β1 antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TGF-β1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Materials provided with the kit