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PRS-01914hu Human Complement Factor B (CFB) ELISA Kit

PRS-01914hu Catalogue No.
Reactivity
Human
Range
30pg/mL -1000pg/mL
Sensitivity
5.0pg/mL
Catalogue NoSpecificationExw Reference Price( USD)Qty Avail
PRS-01914hu48T268In store
PRS-01914hu96T368In store


Purpose

This kit allows for the determination of CFB concentrations in Human serum,  blood plasma, and other biological fluids. Principle of the assay

The kit assay Human CFB level in the sampleuse Purified Human CFB antibody  to coat microtiter plate wells, make solid-phase antibody, then add CFB to wells,  Combined CFB antibody which With HRP labeledbecome antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB  substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the  addition of a sulphuric acid solution and the color change is measured  spectrophotometrically at a wavelength of 450 nm. The concentration of CFB in the  samples is then determined by comparing the O.D. of the samples to the standard curve.