Purpose
This kit allows for the determination of HDL-C concentrations in Human serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Human HDL-C level in the sample，use Purified Human HDL-C antibody to coat microtiter plate wells, make solid-phase antibody, then add HDL-C to wells, Combined HDL-C antibody which With HRP labeled，become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRPenzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the colorchange is measured spectrophotometrically at a wavelength of 450 nm. The concentration of HDLC in the samples is then determined by comparing the O.D. of the samples to the standard curve.
